Effects of 3′-OH and 5′-PO 4 Base Mispairs and Damaged Base Lesions on the Fidelity of Nick Sealing by Deinococcus radiodurans RNA Ligase

Author:

Schmier Brad J.1,Shuman Stewart1

Affiliation:

1. Molecular Biology Program, Sloan-Kettering Institute, New York, New York, USA

Abstract

ABSTRACT Deinococcus radiodurans RNA ligase (DraRnl) is the founding member of a family of end-joining enzymes encoded by diverse microbes and viruses. DraRnl ligates 3′-OH, 5′-PO 4 nicks in double-stranded nucleic acids in which the nick 3′-OH end is RNA. Here we gauge the effects of 3′-OH and 5′-PO 4 base mispairs and damaged base lesions on the rate of nick sealing. DraRnl is indifferent to the identity of the 3′-OH nucleobase, provided that it is correctly paired. With 3′-OH mispairs, the DraRnl sealing rate varies widely, with G-T and A-C mispairs being the best substrates and G-G, G-A, and A-A mispairs being the worst. DraRnl accepts 3′ A–8-oxoguanine (oxoG) to be correctly paired, while it discriminates against U-oxoG and G-oxoG mispairs. DraRnl displays high activity and low fidelity in sealing 3′-OH ends opposite an 8-oxoadenine lesion. It prefers 3′-OH adenosine when sealing opposite an abasic template site. With 5′-PO 4 mispairs, DraRnl seals a 5′ T-G mispair as well as it does a 5′ C-G pair; in most other respects, the ligation fidelity at 5′ mispairs is similar to that at 3′ mispairs. DraRnl accepts a 5′ A-oxoG end to be correctly paired, yet it is more tolerant of 5′ T-oxoG and 5′ G-oxoG mispairs than the equivalent configurations on the 3′ side of the nick. At 5′ nucleobase-abasic site nicks, DraRnl prefers to ligate when the nucleobase is a purine. The biochemical properties of DraRnl are compatible with its participation in the templated repair of RNA damage or in the sealing of filled DNA gaps that have a 3′ ribopatch.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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