A single-molecule sequencing assay for the comprehensive profiling of T4 DNA ligase fidelity and bias during DNA end-joining-Reference-Cited by-同舟云学术

A single-molecule sequencing assay for the comprehensive profiling of T4 DNA ligase fidelity and bias during DNA end-joining

Published:2018-05-08 Issue:13 Volume:46 Page:e79-e79
ISSN:0305-1048
Container-title:Nucleic Acids Research
language:en
Short-container-title:

Author:

Potapov Vladimir¹,Ong Jennifer L¹,Langhorst Bradley W²,Bilotti Katharina¹,Cahoon Dan³,Canton Barry³,Knight Thomas F³,Evans Thomas C¹,Lohman Gregory J S¹

Affiliation:

1. Research Department, New England Biolabs, Ipswich, MA 01938, USA

2. Applications and Product Development, New England Biolabs, Ipswich, MA 01938, USA

3. Ginkgo Bioworks, Boston, MA 02210, USA

Publisher

Oxford University Press (OUP)

Subject

Genetics

Link

http://academic.oup.com/nar/article-pdf/46/13/e79/25227949/gky303.pdf

Reference37 articles.

1. DNA ligases: structure, reaction mechanism, and function;Tomkinson;Chem. Rev.,2006

2. DNA and RNA ligases: structural variations and shared mechanisms;Pascal;Curr. Opin. Struct. Biol.,2008

3. DNA ligases: progress and prospects;Shuman;J. Biol. Chem.,2009

4. Sealing of gaps in duplex DNA by T4 DNA ligase;Nilsson;Nucleic Acids Res.,1982

5. Nicks 3′ or 5′ to AP sites or to mispaired bases, and one-nucleotide gaps can be sealed by T4 DNA ligase;Goffin;Nucleic Acids Res.,1987

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