Comparative Genomics of Cyanobacterial Symbionts Reveals Distinct, Specialized Metabolism in Tropical Dysideidae Sponges-Reference-Cited by-同舟云学术

Comparative Genomics of Cyanobacterial Symbionts Reveals Distinct, Specialized Metabolism in Tropical Dysideidae Sponges

Published:2019-06-25 Issue:3 Volume:10 Page:
ISSN:2161-2129
Container-title:mBio
language:en
Short-container-title:mBio

Author:

Schorn Michelle A.¹,Jordan Peter A.¹,Podell Sheila¹,Blanton Jessica M.¹,Agarwal Vinayak¹²,Biggs Jason S.³,Allen Eric E.¹⁴⁵,Moore Bradley S.¹⁴⁶^ORCID

Affiliation:

1. Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, California, USA

2. School of Chemistry and Biochemistry, School of Biological Sciences, Georgia Institute of Technology, Atlanta, Georgia, USA

3. University of Guam Marine Laboratory, UoG Station, Mangilao, Guam, USA

4. Center for Microbiome Innovation, University of California, San Diego, California, USA

5. Division of Biological Sciences, University of California, San Diego, California, USA

6. Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, California, USA

Abstract

Natural products provide the inspiration for most clinical drugs. With the rise in antibiotic resistance, it is imperative to discover new sources of chemical diversity. Bacteria living in symbiosis with marine invertebrates have emerged as an untapped source of natural chemistry. While symbiotic bacteria are often recalcitrant to growth in the lab, advances in metagenomic sequencing and assembly now make it possible to access their genetic blueprint. A cell enrichment procedure, combined with a hybrid sequencing and assembly approach, enabled detailed genomic analysis of uncultivated cyanobacterial symbiont populations in two chemically rich tropical marine sponges. These population genomes reveal a wealth of secondary metabolism potential as well as possible reasons for historical difficulties in their cultivation.

Funder

National Science Foundation

HHS | NIH | National Institute of Environmental Health Sciences

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mBio.00821-19

Reference79 articles.

1. Natural Products as Sources of New Drugs from 1981 to 2014

2. New marine natural products from sponges (Porifera) of the order Dictyoceratida (2001 to 2012); a promising source for drug discovery, exploration and future prospects