Bacillus subtilis mutant deficient in the ability to produce the dipeptide antibiotic bacilysin: isolation and mapping of the mutation

Author:

Hilton M D1,Alaeddinoglu N G1,Demain A L1

Affiliation:

1. Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge 02139.

Abstract

A Bacillus subtilis mutant which carries a lesion in a gene specific to the synthesis of the dipeptide antibiotic bacilysin was isolated. A derivative strain in which transposon Tn917 had inserted near the bacilysin lesion was isolated and used as the donor in PBS-1 transduction mapping experiments. The bac-1 locus was mapped between the ctrA and sacA loci, near 90% on the standard B. subtilis 168 chromosome map.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference11 articles.

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2. Efficient Bacillus subtilis cloning system using bacteriophage vector 4105J9;Errington J.;J. Gen. Microbiol.,1984

3. Synthesis of bacilysin by Bacillus subtilis branches from prephenate of the aromatic amino acid pathway;Hilton M. D.;J. Bacteriol.,1988

4. Transformation and transduction in recombination-defective mutants of Bacillus subtilis;Hoch J. A.;J. Bacteriol.,1967

5. Miller J. H. 1972. Experiments in molecular genetics p. 125-129. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.

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