PURIFICATION AND MOLECULAR WEIGHT DETERMINATION OF CLOSTRIDIUM BOTULINUM TYPE E TOXIN

Author:

Gerwing Julia1,Dolman Claude E.1,Reichmann M. E.1,Bains Hardial S.1

Affiliation:

1. Department of Bacteriology and Immunology, The University of British Columbia, and The Federal Department of Agriculture Research Station, Vancouver, British Columbia, Canada

Abstract

Gerwing, Julia (The University of British Columbia, Vancouver, British Columbia, Canada), Claude E. Dolman, M. E. Reichmann, and Hardial S. Bains . Purification and molecular weight determination of Clostridium botulinum type E toxin. J. Bacteriol. 88: 216–219. 1964.—A method was developed whereby type E botulinus toxin can be obtained in a highly purified state by elution through acidified diethylaminoethyl-cellulose columns. The material thus isolated appears to be electrophoretically and ultracentrifugally homogeneous. A molecular weight of 18,600 was calculated for the toxin.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference16 articles.

1. Clostridium botulinum type E;DOLMAN C. E.;Intern. Congr. Microbiol. 6th Rome,1953

2. Recent observations on type E botulism;DOLMAN C. E.;Can. J. Public Health,1957

3. Activation of Clostridium botulintum type E toxins by trypsin;DUFF J. T.;J. Bacteriol.,1956

4. Purification and activation of Clostridium botulinunt type E toxin;GERWING J., C.;J. Bacteriol.,1961

5. Activation phenomenon of Clostridium botulinum type E toxin;GERWING J., C.;J. Bacteriol.,1962

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