Affiliation:
1. Roche Vitamins Inc., Nutley, New Jersey 07110
Abstract
ABSTRACT
We have developed an antisense oligonucleotide microarray for the study of gene expression and regulation in
Bacillus subtilis
by using Affymetrix technology. Quality control tests of the
B. subtilis
GeneChip were performed to ascertain the quality of the array. These tests included optimization of the labeling and hybridization conditions, determination of the linear dynamic range of gene expression levels, and assessment of differential gene expression patterns of known vitamin biosynthetic genes. In minimal medium, we detected transcripts for approximately 70% of the known open reading frames (ORFs). In addition, we were able to monitor the transcript level of known biosynthetic genes regulated by riboflavin, biotin, or thiamine. Moreover, novel transcripts were also detected within intergenic regions and on the opposite coding strand of known ORFs. Several of these novel transcripts were subsequently correlated to new coding regions.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
88 articles.
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