Enhanced Broth Media for Selective Growth of Vibrio vulnificus

Author:

Hsu Wei-Yea12,Wei Cheng-i1,Tamplin Mark L.12

Affiliation:

1. Department of Food Science and Human Nutrition1 and

2. Department of Family, Youth & Community Sciences,2 University of Florida, Gainesville, Florida 32611

Abstract

ABSTRACT Rapid detection of Vibrio vulnificus can be enhanced by optimizing the components of enrichment broth. PNC (5% peptone, 1% NaCl, and 0.08% cellobiose [pH 8.0]) enhanced the growth of V. vulnificus compared to alkaline peptone broth. PNCC (PNC with 1.0 to 4.1 U of colistin methanesulfonate per ml) increased the growth of low levels of V. vulnificus while suppressing non-target bacteria.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference17 articles.

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3. Elliot E. L. Kaysner C. A. Tamplin M. L. V. cholerae V. parahaemolyticus V. vulnificus and other Vibrio spp. U.S. Food and Drug Administration Bacteriological analytical manual 7th ed. 1992 111 140 AOAC International Arlington Va

4. Hill W. E. Datta A. R. Feng P. Lampel K. A. Payne W. L. Identification of foodborne bacterial pathogens by gene probes U.S. Food and Drug Administration Bacteriological analytical manual 7th ed. 1992 383 417 AOAC International Arlington Va

5. Polymerase chain reaction identification of Vibrio vulnificus in artificially contaminated oysters

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