Improved 2α-Hydroxylation Efficiency of Steroids by CYP154C2 Using Structure-Guided Rational Design

Author:

Gao Qilin1,Ma Bingbing2,Wang Qianwen3,Zhang Hao4,Fushinobu Shinya56,Yang Jian1,Lin Susu1,Sun Keke1,Han Bing-Nan1,Xu Lian-Hua1ORCID

Affiliation:

1. College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China

2. Research Center for Clinical Pharmacy, The First Affiliated Hospital & Institute of Pharmaceutical Biotechnology, School of Medicine, Zhejiang University, Hangzhou, China

3. Ocean College, Zhejiang University, Zhoushan, China

4. Drug Discovery and Design Center, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China

5. Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo, Japan

6. Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Tokyo, Japan

Abstract

Hydroxylated derivatives of steroids play essential roles in medicine. Cytochrome P450 enzymes selectively hydroxylate methyne groups on steroids, which can dramatically change their polarity, biological activity and toxicity.

Funder

NSFC | NSFC-Zhejiang Joint Fund | 浙江省科学技术厅 | Natural Science Foundation of Zhejiang Province

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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