Affiliation:
1. Kemin Industries (Asia) Pte Ltd., 12 Senoko Drive, Singapore 758200, Republic of Singapore
Abstract
ABSTRACT
The objectives of this study were to isolate beneficial strains of microorganisms from the gastrointestinal tracts of healthy chickens and to screen them against
Clostridium perfringens
, a causative agent of necrotic enteritis in poultry. One of the bacteria isolated, a strain of
Bacillus subtilis
, was found to possess an anticlostridial factor that could inhibit the
C. perfringens
ATCC 13124 used in this study. The anticlostridial factor produced by
B. subtilis
PB6 was found to be fully or partially inactivated in the presence of pronase, trypsin, and pepsin. In contrast, the antimicrobial activity of the anticlostridial factor was not affected by treatment at 100 or 121°C or by treatment with any of the organic solvents used in the study. The optimum growth temperature and optimum pH for production of the anticlostridial factor were 37°C and 6.20, respectively. Using the mass spectroscopy-mass spectroscopy technique, the apparent molecular mass of the anticlostridial factor was estimated to be in the range from 960 to 983 Da. In terms of the antimicrobial spectrum, the anticlostridial factor was inhibitory toward various strains of
C. perfringens
implicated in necrotic enteritis in poultry,
Clostridium difficile
,
Streptococcus pneumoniae
,
Campylobacter jejuni
, and
Campylobacter coli.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference41 articles.
1. Persistence of Colonization of Human Colonic Mucosa by a Probiotic Strain,
Lactobacillus rhamnosus
GG, after Oral Consumption
2. Baquero, F., and F. Moreno. 1984. The microcins. FEMS Microbiol. Lett.23:117-124.
3. Cordovilla, P., E. Valdivia, A. Gonzalez-Segura, A. Galvez, M. Martinez-Bueno, and M. Maqueda. 1993. Antagonistic action of the bacterium Bacillus licheniformis M-4 toward amoeba Naegleria fowleri. J. Eukaryot. Microbiol.323-328.
4. Cowen, B. S., L. D. Schwartz, R. A. Wilson, and S. I. Ambrus. 1987. Experimentally induced necrotic enteritis in chickens. Avian Dis.31:904-906.
5. Craven, S. E., N. J. Stern, N. A. Cox, J. S. Bailey, and M. Berrang. 1999. Cecal carriage of Clostridium perfringens in broiler chicken given Mucosal Starter Culture. Avian Dis.43:484-490.
Cited by
130 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献