Identification of a Rough Strain of Escherichia coli O157:H7 That Produces No Detectable O157 Antigen

Author:

Feng Peter1,Sandlin Robin C.2,Park Choong H.3,Wilson Richard A.4,Nishibuchi Mitsuaki5

Affiliation:

1. Division of Microbiological Studies, Food and Drug Administration, Washington, D.C. 202041;

2. Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 208142;

3. INOVA Fairfax Hospital, Falls Church, Virginia 220423;

4. Escherichia coli Reference Center, Pennsylvania State University, University Park, Pennsylvania 168024; and

5. Center for Southeast Asian Studies, Kyoto University, Kyoto, Japan 606-015

Abstract

ABSTRACT MA6, an O157:H7-like strain, did not react with most anti-O157 kits examined; however, it had the rfbE gene that is essential for O157 expression and carried O157:H7 virulence factors. Lipopolysaccharide analysis showed that MA6 is a rough strain that does not produce the O157 antigen, but genetically, it belongs in the O157:H7 clonal group.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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