Multiplex PCR Assay for Rapid Detection and Genotyping of Helicobacter pylori Directly from Biopsy Specimens-Reference-Cited by-同舟云学术

Multiplex PCR Assay for Rapid Detection and Genotyping of Helicobacter pylori Directly from Biopsy Specimens

Published:2004-06 Issue:6 Volume:42 Page:2821-2824
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Chattopadhyay Santanu¹,Patra Rajashree¹,Ramamurthy T.¹,Chowdhury Abhijit²,Santra Amal²,Dhali G. K.²,Bhattacharya S. K.¹,Berg Douglas E.³,Nair G. Balakrish⁴,Mukhopadhyay Asish K.¹

Affiliation:

1. National Institute of Cholera and Enteric Diseases

2. Department of Gastroenterology, Institute of Post Graduate Medical Education and Research, Calcutta, India

3. Department of Molecular Microbiology, Washington University Medical School, St. Louis, Missouri 63110

4. International Centre for Diarrhoeal Diseases Research, Dhaka, Bangladesh

Abstract

ABSTRACT We developed and evaluated a simple, novel multiplex PCR assay for rapid detection of Helicobacter pylori infection and for the determination of vacA and cagA genotypes directly from gastric biopsy specimens. This assay did not require culturing of strains or extraction of DNA from biopsy samples. This multiplex PCR assay would be of particularly great value for laboratories in developing countries.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.6.2821-2824.2004

Reference15 articles.

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