Affiliation:
1. Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, CSIC, Campus de la Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain
Abstract
ABSTRACT
Oxidation of
n
-alkanes in bacteria is normally initiated by an enzyme system formed by a membrane-bound alkane hydroxylase and two soluble proteins, rubredoxin and rubredoxin reductase.
Pseudomonas aeruginosa
strains PAO1 and RR1 contain genes encoding two alkane hydroxylases (
alkB1
and
alkB2
), two rubredoxins (
alkG1
and
alkG2
), and a rubredoxin reductase (
alkT
). We have localized the promoters for these genes and analyzed their expression under different conditions. The
alkB1
and
alkB2
genes were preferentially expressed at different moments of the growth phase; expression of
alkB2
was highest during the early exponential phase, while
alkB1
was induced at the late exponential phase, when the growth rate decreased. Both genes were induced by C
10
to C
22
/C
24
alkanes but not by their oxidation derivatives. However, the
alkG1
,
alkG2
, and
alkT
genes were expressed at constant levels in both the absence and presence of alkanes.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
83 articles.
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