Heat-induced requirements for sucrose or magnesium for expression of heat resistance in Bacillus cereus forespores

Author:

Busta F F,Baillie E,Murrell W G

Abstract

The addition of 0.6 M sucrose of 0.016 M Mg2+ to the enumeration medium was required for early expression of heat resistance (10 min at 70 degrees C) in stage V Bacillus cereus forspores. The addition of Mg2+ to the sporulation medium did not remove this requirment for sucrose of Mg2+. The heat damage did not affect forespore germination or outgrowth, but injured cells in the absence of sucrose or Mg2+ were not capable of cell division. The heat-induced sublethal damage apparently affected the forspore component(s) that could be repaired or was capable of normal function in the presence of added Mg2+ or sucrose.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference16 articles.

1. Requirement for and sensitivity to lysozyme by Clostridium perfringens spores heated at ultrahigh temperatures;Adams D. M.;Appl. Microbiol.,1974

2. Adams D. M. and F. F. Busta. 1972. Heat injury as the selective inactivation of a Bacillus subtilis spore ger- mination system p. 368-377. In H. 0. Halvorson R. Hanson and L. L. Campbell (ed.) Spores V. American Society for Microbiology Washington D.C.

3. Ultrahigh-temperature activation of a low-temperature Bacillus subtilis spore germination system;Adams D. M.;Appl. Microbiol.,1972

4. Effect of lysozyme on the recovery of heated Clostridium botulinum spores;Alderton G.;Appl. Microbiol.,1974

5. Repair of heat-injured Clostridium perfringens spores during outgrowth;Barach J. T.;Appl. Microbiol.,1975

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