Major Role of NAD-Dependent Lactate Dehydrogenases in Aerobic Lactate Utilization in Lactobacillus plantarum during Early Stationary Phase-Reference-Cited by-同舟云学术

Major Role of NAD-Dependent Lactate Dehydrogenases in Aerobic Lactate Utilization in Lactobacillus plantarum during Early Stationary Phase

Published:2004-10 Issue:19 Volume:186 Page:6661-6666
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Goffin Philippe¹,Lorquet Frédérique¹,Kleerebezem Michiel²,Hols Pascal¹

Affiliation:

1. Unité de Génétique, Institut des Sciences de la Vie, Université Catholique de Louvain, Louvain-la-Neuve, Belgium

2. Wageningen Centre for Food Sciences, NIZO Food Research, Ede, The Netherlands

Abstract

ABSTRACT NAD-independent lactate dehydrogenases are commonly thought to be responsible for lactate utilization during the stationary phase of aerobic growth in Lactobacillus plantarum . To substantiate this view, we constructed single and double knockout mutants for the corresponding genes, loxD and loxL . Lactate-to-acetate conversion was not impaired in these strains, while it was completely blocked in mutants deficient in NAD-dependent lactate dehydrogenase activities, encoded by the ldhD and ldhL genes. We conclude that NAD-dependent but not NAD-independent lactate dehydrogenases are involved in this process.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.186.19.6661-6666.2004

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