Use of Molecular Beacons To Detect an Antifolate Resistance-Associated Mutation in Plasmodium falciparum-Reference-Cited by-同舟云学术

Use of Molecular Beacons To Detect an Antifolate Resistance-Associated Mutation in Plasmodium falciparum

Published:2000-12 Issue:12 Volume:44 Page:3461-3464
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Durand Rémy¹,Eslahpazire Jobin¹,Jafari Sayeh¹,Delabre Jean-François¹,Marmorat-Khuong Anne¹,di Piazza Jean-Philippe¹,Le Bras Jacques¹

Affiliation:

1. Centre National de Référence pour la Chimiosensibilité du Paludisme, Université Paris V et Assistance Publique-Hôpitaux de Paris, Hôpital Bichat-Claude Bernard, Laboratoire de Parasitologie, 75877 Paris Cedex 18, France

Abstract

ABSTRACT A PCR-based technique using new fluorescent probes, called molecular beacons, was developed to detect the antifolate resistance-associated S108N point mutation in Plasmodium falciparum . One hundred African clinical isolates were tested by the new method in comparison with the PCR-restriction fragment length polymorphism method. This new molecular technique appears to be a promising tool for epidemiological studies.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.44.12.3461-3464.2000

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