Using Species a Rotavirus Reverse Genetics to Engineer Chimeric Viruses Expressing SARS-CoV-2 Spike Epitopes-Reference-Cited by-同舟云学术

Using Species a Rotavirus Reverse Genetics to Engineer Chimeric Viruses Expressing SARS-CoV-2 Spike Epitopes

Published:2022-07-27 Issue:14 Volume:96 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Diebold Ola¹,Gonzalez Victoria¹,Venditti Luca²,Sharp Colin¹,Blake Rosemary A.¹,Tan Wenfang S.¹,Stevens Joanne¹,Caddy Sarah³,Digard Paul¹^ORCID,Borodavka Alexander²,Gaunt Eleanor¹^ORCID

Affiliation:

1. Infection and Immunity Division, Roslin Institute, University of Edinburgh, Easter Bush Campus, Midlothian, United Kingdom

2. Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom

3. Cambridge Institute of Therapeutic Immunology and Infectious Disease, Department of Medicine, University of Cambridge, Cambridge, United Kingdom

Abstract

We explored the use of rotaviruses (RVs) to express heterologous peptides, using SARS-CoV-2 as an example. Small SARS-CoV-2 peptide insertions (<34 amino acids) into the hypervariable region of the viral protein 4 (VP4) of RV SA11 strain resulted in reduced viral titer and replication, demonstrating a limited tolerance for peptide insertions at this site.

Funder

UKRI | Biotechnology and Biological Sciences Research Council

Wellcome Trust

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.00488-22

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