Characterization of In Vitro Chlamydial Cultures in Low-Oxygen Atmospheres

Author:

Juul Nicolai1,Jensen Helene12,Hvid Malene1,Christiansen Gunna1,Birkelund Svend1

Affiliation:

1. Institute of Medical Microbiology and Immunology

2. Institute of Biology, University of Aarhus, Aarhus, Denmark

Abstract

ABSTRACT To mimic in vivo conditions during chlamydial infections, Chlamydia trachomatis serovar D and Chlamydia pneumoniae CWL029 were cultured in low-oxygen atmospheres containing 4% O 2 , with parallel controls cultured in atmospheric air. Both were enriched with 5% CO 2 . The results showed a dramatic increase in the growth of C. pneumoniae but not of C. trachomatis .

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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4. Gorczynski, R. J., and B. R. Duling. 1978. Role of oxygen in arteriolar functional vasodilation in hamster striated muscle. Am. J. Physiol. 235 : H505-H515.

5. Hahn, D. L., A. A. Azenabor, W. L. Beatty, and G. I. Byrne. 2002. Chlamydia pneumoniae as a respiratory pathogen. Front. Biosci. 7 : e66-76.

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