Evaluation of Histoplasmin for the Presence of H and M Antigens: Some Difficulties Encountered in the Production and Evaluation of a Product Suitable for the Immunodiffusion Test

Author:

Gross Herman1,Bradley Georgia1,Pine Leo1,Gray Sam1,Green James H.1,Harrell W. Knox1

Affiliation:

1. Center for Disease Control, Atlanta, Georgia 30333

Abstract

The development of a single histoplasmin suitable for use in immunodiffusion testing of a variety of human histoplasmosis sera depends not only upon the presence of both antigens, but on relative concentration of one to the other, and perhaps on the chemical characteristics of the H and M antigens of a given strain.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference9 articles.

1. Purification, composition, and serological characterization of histoplasmin H and M antigens;Bradley G.;Infect. Immunity,1974

2. Factors influencing the production of H and M antigens by Histoplasma capsulatum: development and evaluation of a shake culture procedure;Ehrhard H. B.;Appl. Microbiol.,1972

3. Factors influencing the production of H and M antigen by Histoplasma capsulatum: effect of physical factors and composition of medium;Ehrhard H. B.;Appl. Microbiol.,1972

4. Harrell W. (ed.) 1973. Preparation of Histoplasma capsulatum (Histoplasmin) CF antigen p. 98-100. In Procedural manual for production of bacterial fungal and parasitic reagents. Center for Disease Control Atlanta Georgia.

5. Value of immunodiffusion tests in the diagnosis of systemic mycotic diseases;Kaufman L.;Ann. Clin. Lab. Sci.,1973

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