Factors Influencing the Production of H and M Antigens by Histoplasma capsulatum : Development and Evaluation of a Shake Culture Procedure-Reference-Cited by-同舟云学术

Factors Influencing the Production of H and M Antigens by Histoplasma capsulatum : Development and Evaluation of a Shake Culture Procedure

Published:1972-02 Issue:2 Volume:23 Page:236-249
ISSN:0003-6919
Container-title:Applied Microbiology
language:en
Short-container-title:Appl Microbiol

Author:

Ehrhard Hugh-Bert¹²,Pine Leo¹²

Affiliation:

1. Department of Parasitology and Laboratory Practice, School of Public Health, University of North Carolina, Chapel Hill, North Carolina 27515

2. Center for Disease Control, Atlanta, Georgia 30333

Abstract

Studies were undertaken to improve the production of histoplasmin for use in complement-fixation tests and in the determination of H and M antibodies. A shake culture method performed at 25 C was developed with a yeast-phase inoculum. Eight strains of Histoplasma were tested in three synthetic media to evaluate the effects of strain and medium on H and M antigen production. Intrastrain variation was negligible, and antigen production was reproducible. All of the strains produced H antigen; six strains produced both H and M antigens, and two produced only H antigen. The time of H and M antigen appearance varied with the medium; M antigen appearance was dependent upon the strain and medium used. Titers of M antigen appeared to be greater in stagnant culture.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://journals.asm.org/doi/pdf/10.1128/am.23.2.236-249.1972

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3. Specific immunological test for the rapid identification of members of the genus Histoplasma;Journal of Clinical Microbiology;1976-02

4. Evaluation of Histoplasmin for the Presence of H and M Antigens: Some Difficulties Encountered in the Production and Evaluation of a Product Suitable for the Immunodiffusion Test;Journal of Clinical Microbiology;1975-03

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