Construction of a cosmid clone library of Pseudomonas syringae pv. phaseolicola and isolation of genes by functional complementation-Reference-Cited by-同舟云学术

Construction of a cosmid clone library of Pseudomonas syringae pv. phaseolicola and isolation of genes by functional complementation

Published:1985-07 Issue:1 Volume:50 Page:169-171
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Ehrenshaft M,Mills D

Abstract

A genomic library constructed from a wild-type strain of Pseudomonas syringae pv. phaseolicola in the broad-host-range cosmid vector pVK102 was used to isolate wild-type genes by complementation of Tn5-induced auxotrophic mutants. Selection pressure was required for maintenance of the vector and members of the library in strains of P. syringae.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.50.1.169-171.1985

Reference15 articles.

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5. Cloning of genes involved in pathogenicity of Xanthomonas campestris pv. campestris using the broad host range cosmid pLAFR1;Daniels M. J.;EMBO J.,1984

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3. Construction of a stable shuttle vector for high-frequency transformation in Pseudomonas syringae pv. syringae;Journal of Bacteriology;1990-01

4. Identification and mapping of regions that confer plasmid functions and of sites for excisive recombination of plasmid pMMC7105;Molecular and General Genetics MGG;1987-02

5. Molecular Analysis of Pathogenicity of Pseudomonas Syringae Pathovars Phaseolicola and Syringae;Plant Pathogenic Bacteria;1987