Construction of a stable shuttle vector for high-frequency transformation in Pseudomonas syringae pv. syringae-Reference-Cited by-同舟云学术

Construction of a stable shuttle vector for high-frequency transformation in Pseudomonas syringae pv. syringae

Published:1990-01 Issue:1 Volume:172 Page:477-480
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Mukhopadhyay P¹,Mukhopadhyay M¹,Mills D¹

Affiliation:

1. Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331.

Abstract

A cryptic 80.3-kilobase plasmid, pOSU900, in Pseudomonas syringae pv. syringae strain J900 could be cured by treatment with mitomycin without affecting the pathogenicity of J900 on the host, Phaseolus vulgaris L. The replication region of pOSU900 was identified, subcloned, and modified for construction of a high-copy cloning vector. This vector could be transformed into Pseudomonas strains with high efficiency (ca. 10(6) transformants per microgram of DNA) and was very stable during growth of the host bacteria in planta.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.172.1.477-480.1990

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