Author:
Tessaro Michael J.,Soliman Sameh S. M.,Raizada Manish N.
Abstract
ABSTRACTA whole-cell biosensor for glutamine (GlnLux) was constructed by transforming anEscherichia coliglutamine (Gln) auxotroph with a constitutiveluxreporter gene. Measurements of Gln in plant extracts using GlnLux correlated with quantification using high-performance liquid chromatography (Spearman'sr= 0.95). GlnLux permitted charge-coupled-device (CCD) imaging of Gln from whole plant organs.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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