Random Mutagenesis of Clostridium cellulolyticum by Using a Tn 1545 Derivative-Reference-Cited by-同舟云学术

Random Mutagenesis of Clostridium cellulolyticum by Using a Tn 1545 Derivative

Published:2010-07 Issue:13 Volume:76 Page:4546-4549
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Blouzard Jean-Charles¹²,Valette Odile¹,Tardif Chantal¹²,de Philip Pascale¹²

Affiliation:

1. Laboratoire de Chimie Bactérienne, IFR88-CNRS, Marseille, France

2. Université d'Aix-Marseille, Marseille, France

Abstract

ABSTRACT Further understanding of the plant cell wall degradation system of Clostridium cellulolyticum and the possibility of metabolic engineering in this species highlight the need for a means of random mutagenesis. Here, we report the construction of a Tn 1545 -derived delivery tool which allows monocopy random insertion within the genome.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02417-09

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5. Cellulase, Clostridia, and Ethanol

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