Simple Histidine-Rich Protein 2 Double-Site Sandwich Enzyme-Linked Immunosorbent Assay for Use in Malaria Drug Sensitivity Testing-Reference-Cited by-同舟云学术

Simple Histidine-Rich Protein 2 Double-Site Sandwich Enzyme-Linked Immunosorbent Assay for Use in Malaria Drug Sensitivity Testing

Published:2005-08 Issue:8 Volume:49 Page:3575-3577
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Noedl Harald¹²,Bronnert Jan³,Yingyuen Kritsanai¹,Attlmayr Bernhard²,Kollaritsch Herwig²,Fukuda Mark¹

Affiliation:

1. Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, 316/6 Rajvithi Road, Bangkok 10400, Thailand

2. Department of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Kinderspitalgasse 15, A-1095 Vienna, Austria

3. Angewandte Tropenmedizin und Infektionsepidemiologie, Schiffahrtmedizinisches Institut der Marine, Fachgebiet I 3, Kopperpahler Allee 120, 24119 Kronshagen, Germany

Abstract

ABSTRACT A simple double-site sandwich enzyme-linked immunosorbent assay (ELISA) for Plasmodium falciparum in vitro drug sensitivity tests based on measuring histidine-rich protein 2 (HRP2) is presented. The ELISA uses two commercial monoclonal antibodies and provides a drastically cheaper alternative to the test kits previously used in the HRP2 drug sensitivity test. The assay is simple to establish and perform. The sensitivity is comparable and the drug sensitivity results very closely match those obtained with the commercial ELISA kits ( R 2 = 0.979; P < 0.001; mean log difference at the 50% inhibitory concentration = 0.07).

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.49.8.3575-3577.2005

Reference6 articles.

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