Author:
Trinel P A,Bleuze P,Leroy G,Moschetto Y,Leclerc H
Abstract
The described apparatus, carrying 190 tubes, allows automatic and aseptic dilution of liquid or suspended-solid samples. Serial 10-fold dilutions are programmable from 10(-1) to 10(-9) and are carried out in glass tubes with screw caps and split silicone septa. Dilution assays performed with strains of Escherichia coli and Bacillus stearothermophilus permitted efficient conditions for sterilization of the needle to be defined and showed that the automatic dilutions were as accurate and as reproducible as the most rigorous conventional dilutions.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
2 articles.
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1. Micropipette use for decimal dilution of bacteriological samples;Annales de l'Institut Pasteur / Microbiologie;1986-01
2. Overview of Rapid Microbiological Methods;Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems