Affiliation:
1. Laboratoire de Microbiologie, US, INRA, ENSBANA, Université de Bourgogne, Dijon, France. cavinjf@u-bourgogne.fr
Abstract
By using degenerate primers designed from the first 19 N-terminal amino acids of Lactobacillus plantarum p-coumaric acid decarboxylase (PDC), a 56-bp fragment was amplified from L. plantarum in PCRs and used as a probe for screening an L. plantarum genomic bank. Of the 2,880 clones in the genomic bank, one was isolated by colony hybridization and contained a 519-bp open reading frame (pdc gene) followed by a putative terminator structure. The pdc gene is expressed on a monocistronic transcriptional unit, which is transcribed from promoter sequences homologous to Lactococcus promoter sequences. No mRNA from pdc and no PDC activity were detected in uninduced cell extracts, indicating that the expression is transcriptionally regulated by p-coumaric acid, which corresponds to an activation factor up to 6,000. The pdc gene was overexpressed constitutively in Escherichia coli, and the recombinant enzyme was purified and characterized.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference30 articles.
1. La décarboxylation des acides cinnamiques substitués par les levures;Albagnac G.;Ann. Technol. Agric.,1975
2. Metabolism of ferulic acid by a facultatively anaerobic strain of Pseudomonas cepacia;Andreoni V.;Syst. Appl. Microbiol.,1984
3. Studies on Iysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli;Bertani G.;J. Bacteriol.,1951
4. Ability of wine lactic acid bacteria to metabolize phenol carboxylic acids;Cavin J. F.;Am. J. Enol. Vitic.,1993
5. Purification and characterization of an inducible p-coumaric acid decarboxylase from Lactobacillus plantarum;Cavin J. F.;FEMS Microbiol Lett.,1997
Cited by
121 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献