Gene cloning, purification, and characterization of a heat-stable phytase from the fungus Aspergillus fumigatus-Reference-Cited by-同舟云学术

Gene cloning, purification, and characterization of a heat-stable phytase from the fungus Aspergillus fumigatus

Published:1997-05 Issue:5 Volume:63 Page:1696-1700
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Pasamontes L¹,Haiker M¹,Wyss M¹,Tessier M¹,van Loon A P¹

Affiliation:

1. Vitamins & Fine Chemicals Division, F. Hoffmann-La Roche Ltd., Basel, Switzerland. luis.pasamontes@roche.com

Abstract

The finding of heat-stable enzymes or the engineering of moderately thermostable enzymes into more stable ones by random or site-directed mutagenesis has become a main priority of modern biotechnology. We report here for the first time a heat-stable phytase able to withstand temperatures up to 100 degrees C over a period of 20 min, with a loss of only 10% of the initial enzymatic activity. The gene (phyA) encoding this heat-stable enzyme has been cloned from Aspergillus fumigatus and overexpressed in Aspergillus niger. The enzyme showed high activity with 4-nitrophenyl phosphate at a pH range of 3 to 5 and with phytic acid at a pH range of 2.5 to 7.5.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.63.5.1696-1700.1997

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