Affiliation:
1. Department of Bacterial Immunology, Division of Communicable Disease and Immunology, Walter Reed Army Institute of Research, Washington, D.C.
Abstract
Falkow, Stanley
(Walter Reed Army Institute of Research, Washington, D.C.), J. A.
Wohlhieter, R. V. Citarella, and L. S. Baron
. Transfer of episomic elements to
Proteus
. I. Transfer of F-linked chromosomal determinants. J. Bacteriol.
87:
209–219. 1964.—F-linked
lac
+
genes may be transferred from
Escherichia coli
to several species of
Proteus
by conjugation. Usually the transferred genetic elements are markedly unstable in
Proteus
, but repeated plating permits the selection of relatively stable
Proteus lac
+
strains.
Proteus
strains carrying F-linked
lac
+
markers are heterogenotes and limited donors for
lac
+
. In addition, both the fertility and
lac
+
property may be eliminated from
Proteus
by treatment with acridine orange.
Escherichia
and
Proteus
possess very different overall deoxyribonucleic acid (DNA) base compositions. In CsCl density gradients of DNA extracted from
Proteus lac
+
strains, the acquisition of
Escherichia
genes by
Proteus
may be correlated with the addition of a physically recognizable high molecular weight, native DNA fraction of
Escherichia
base composition.
Proteus lac
+
strains synthesize a β-galactosidase which is indistinguishable from
E. coli
enzyme by several criteria. Despite this specificity, the regulatory functions of
Escherichia lac
+
genes appear to be impaired in
Proteus
.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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