A Complete Library of Amino Acid Alterations at N304 in Streptomyces clavuligerus Deacetoxycephalosporin C Synthase Elucidates the Basis for Enhanced Penicillin Analogue Conversion-Reference-Cited by-同舟云学术

A Complete Library of Amino Acid Alterations at N304 in Streptomyces clavuligerus Deacetoxycephalosporin C Synthase Elucidates the Basis for Enhanced Penicillin Analogue Conversion

Published:2004-01 Issue:1 Volume:70 Page:607-609
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Chin Hong Soon¹²,Goo Kian Sim¹,Sim T. S.¹

Affiliation:

1. Department of Microbiology, Faculty of Medicine

2. Tropical Marine Science Institute, National University of Singapore, Singapore

Abstract

ABSTRACT N304 of Streptomyces clavuligerus deacetoxycephalosporin C synthase was mutagenized to alter its catalytic ability. Given that N304A, N304K, N304L, and N304R mutant enzymes exhibited significant improvements in penicillin analogue conversions, we advocate that replacement of N304 with residues with aliphatic or basic side chains is preferable for engineering of a hypercatalytic enzyme.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.70.1.607-609.2004

Reference12 articles.

1. Chin, H. S., and T. S. Sim. 2002. C-terminus modification of Streptomyces clavuligerus deacetoxycephalosporin C synthase improves catalysis with an expanded substrate specificity. Biochem. Biophys. Res. Commun.295:55-61.

2. Chin, H. S., J. Sim, and T. S. Sim. 2001. Mutation of N304 to leucine in Streptomyces clavuligerus deacetoxycephalosporin C synthase creates an enzyme with increased penicillin analogue conversion. Biochem. Biophys. Res. Commun.287:507-513.

3. Chin, H. S., J. Sim, K. I. Seah, and T. S. Sim. 2003. Deacetoxycephalosporin C synthase isozymes exhibit diverse catalytic activity and substrate specificity. FEMS Microbiol. Lett.218:251-257.

4. Cooper, R. D. G. 1993. The enzyme involved in biosynthesis of penicillin and cephalosporin: their structure and function. Bioorg. Med. Chem.1:1-17.

5. Lee, H. J., M. D. Lloyd, K. Harlos, I. J. Clifton, J. E. Baldwin, and C. J. Schofield. 2001. Kinetic and crystallographic studies on deacetoxycephalosporin C synthase (DAOCS). J. Mol. Biol.308:937-948.

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