Affiliation:
1. Universidad de Buenos Aires, Facultad Ciencias Exactas y Naturales, Departamento de Química Biológica, Buenos Aires, Argentina
Abstract
ABSTRACT
We have previously described a murein hydrolase activity for the surface layer (S-layer) of
Lactobacillus acidophilus
ATCC 4356. Here we show that, in combination with nisin, this S-layer acts synergistically to inhibit the growth of pathogenic Gram-negative
Salmonella enterica
and potential pathogenic Gram-positive bacteria,
Staphylococcus aureus
and
Bacillus cereus.
In addition, bacteriolytic effects were observed for the Gram-positive species tested. We postulate that the S-layer enhances the access of nisin into the cell membrane by enabling it to cross the cell wall, while nisin provides the sudden ion-nonspecific dissipation of the proton motive force required to enhance the S-layer murein hydrolase activity.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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