Isolation and characterization of Rhizobium etli mutants altered in degradation of asparagine-Reference-Cited by-同舟云学术

Isolation and characterization of Rhizobium etli mutants altered in degradation of asparagine

Published:1997-03 Issue:6 Volume:179 Page:2068-2072
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Huerta-Zepeda A¹,Ortuño L¹,Du Pont G¹,Durán S¹,Lloret A¹,Merchant-Larios H¹,Calderón J¹

Affiliation:

1. Departamento de Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México D.F.

Abstract

Rhizobium etli mutants unable to grow on asparagine as the nitrogen and carbon source were isolated. Two kinds of mutants were obtained: AHZ1, with very low levels of aspartase activity, and AHZ7, with low levels of asparaginase and very low levels of aspartase compared to the wild-type strain. R. etli had two asparaginases differentiated by their thermostabilities, electrophoretic mobilities, and modes of regulation. The AHZ mutants nodulated as did the wild-type strain and had nitrogenase levels similar to that of the wild-type strain.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.179.6.2068-2072.1997

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