Affiliation:
1. Departamento de Ecologia Molecular, Universidad Nacional Autónoma de México, Morelos.
Abstract
L-[amide-13N]glutamine in Neurospora crassa is metabolized to [13N]glutamate by glutamate synthase and to [13N]ammonium by the glutamine transaminase-omega-amidase pathway. The [13N]ammonium released is assimilated by glutamate dehydrogenase and glutamine synthetase, confirming the operation of a glutamine cycle. Most of the nitrogen is retained during cycling between glutamate and glutamine.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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