Development of Bacteriocinogenic Strains of Saccharomyces cerevisiae Heterologously Expressing and Secreting the Leaderless Enterocin L50 Peptides L50A and L50B from Enterococcus faecium L50

Abstract

ABSTRACT A segregationally stable expression and secretion vector for Saccharomyces cerevisiae , named pYABD01, was constructed by cloning the yeast gene region encoding the mating pheromone α-factor 1 secretion signal ( MF α 1 s ) into the S. cerevisiae high-copy-number expression vector pYES2. The structural genes of the two leaderless peptides of enterocin L50 (EntL50A and EntL50B) from Enterococcus faecium L50 were cloned, separately ( entL50A or entL50B ) and together ( entL50AB ), into pYABD01 under the control of the galactose-inducible promoter P GAL1 . The generation of recombinant S. cerevisiae strains heterologously expressing and secreting biologically active EntL50A and EntL50B demonstrates the suitability of the MF α 1 s -containing vector pYABD01 to direct processing and secretion of these antimicrobial peptides through the S. cerevisiae Sec system.