Affiliation:
1. Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, and the Department of Clinical Pathology, Clinical Center, National Institutes of Health, Bethesda, Maryland 20014
Abstract
The minimum inhibitory concentration (MIC) of 5-fluorocytosine (5-FC) was determined for 65 isolates ofCryptococcus neoformansby using a twofold serial tube dilution method. The MIC was profoundly influenced by incubation temperature, inoculum size, and duration of incubation. By using a standard set of test conditions, 100% of 49 pretreatment isolates ofC. neoformanswere susceptible to 10 μg of 5-FC per ml or less, and 9 (56%) of 16 isolates recovered during or after 5-FC therapy were massively drug resistant (MIC > 320 μg/ml). With the standard test conditions recommended here, the tube dilution method was found to be both accurate and reproducible, and the results correlated with the treatment status of patients.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
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