Affiliation:
1. Department of Molecular Life Science, Tokai University School of Medicine, Isehara 259-1193, Japan
Abstract
ABSTRACT
We evaluated the roles of the MexAB-OprM efflux pump and β-lactamase in β-lactam resistance in
Pseudomonas aeruginosa
by constructing OprM-deficient, OprM basal level, and OprM fully expressed mutants from β-lactamase-negative, -inducible, and -overexpressed strains. We conclude that, with the notable exception of imipenem, the MexAB-OprM pump contributes significantly to β-lactam resistance in both β-lactamase-negative and β-lactamase-inducible strains, while the contribution of the MexAB-OprM efflux system is negligible in strains with overexpressed β-lactamase. Overexpression of the efflux pump alone contributes to the high level of β-lactam resistance in the absence of β-lactamase.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
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