Author:
Hirai K,Suzue S,Irikura T,Iyobe S,Mitsuhashi S
Abstract
Two genetically distinct classes of norfloxacin-resistant Pseudomonas aeruginosa PAO4009 mutants were isolated spontaneously. Two norfloxacin resistance genes, nfxA and nfxB, were mapped hex-9001 and leu-9005 and between pro-9031 and ilv-9023, respectively, on the P. aeruginosa PAO chromosome. The nfxA gene was shown to be an allele of nalA by transductional analysis with bacteriophage F116L. The nfxB mutant showed a 16-fold increase in resistance to norfloxacin and a slight increase in resistance to nalidixic acid. The nfxB mutant was unique in that it showed hypersusceptibility to beta-lactam and aminoglycoside antibiotics. This mutant had about a threefold-lower rate of norfloxacin uptake than that of the wild-type strain or nfxA mutant. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of outer membrane proteins demonstrated the appearance of a 54,000-dalton protein in the nfxB mutant. These findings suggested that the norfloxacin resistance mechanism in the nfxB mutant might be an alteration in outer membrane permeability to norfloxacin.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Reference42 articles.
1. Outer membrane permeability in Pseudomonas aeruginosa: comparison of a wild-type with an antibiotic-susceptible mutant;Angus B. L.;Antimicrob. Agents Chemother.,1982
2. Studies on the mechanism of action of nalidixic acid;Bourguignon G. L.;Antimicrob. Agents Chemother.,1973
3. Lipopolysaccharide changes in impermeability-type aminoglycoside in Pseudomonas aeruginosa;Bryan L. E.;Antimicrob. Agents Chemother.,1984
4. Apparent absence of transferable resistance to nalidixic acid in pathogenic gram-negative bacteria;Burman L. G.;J. Antimicrob. Chemother.,1977
5. New structureactivity relationships of the quinolone antibacterials using the target enzyme. The development and application of a DNA gyrase assay;Domagala J. M.;J. Mol. Chem.,1986
Cited by
199 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献