Genetic Variation among Endosymbionts of Widely Distributed Vestimentiferan Tubeworms

Author:

Di Meo Carol A.1,Wilbur Ami E.2,Holben William E.3,Feldman Robert A.4,Vrijenhoek Robert C.5,Cary S. Craig1

Affiliation:

1. Graduate College of Marine Studies, University of Delaware, Lewes, Delaware 199581;

2. Florida Marine Research Institute, St. Petersburg, Florida 337012;

3. Division of Biological Sciences, The University of Montana, Missoula, Montana 59812-10023;

4. Molecular Dynamics, Sunnyvale, California 94086-45204; and

5. Center for Theoretical and Applied Genetics, Cook College, Rutgers University, New Brunswick, New Jersey 08903-02315

Abstract

ABSTRACT Vestimentiferan tubeworms thriving in sulfidic deep-sea hydrothermal vents and cold seeps are constrained by their nutritional reliance on chemoautotrophic endosymbionts. In a recent phylogenetic study using 16S ribosomal DNA, we found that endosymbionts from vent and seep habitats form two distinct clades with little variation within each clade. In the present study, we used two different approaches to assess the genetic variation among biogeographically distinct vestimentiferan symbionts. DNA sequences were obtained for the noncoding, internal transcribed spacer (ITS) regions of the rRNA operons of symbionts associated with six different genera of vestimentiferan tubeworms. ITS sequences from endosymbionts of host genera collected from different habitats and widely distributed vent sites were surprisingly conserved. Because the ITS region was not sufficient for distinguishing endosymbionts from different habitats or locations, we used a DNA fingerprinting technique, repetitive-extragenic-palindrome PCR (REP-PCR), to reveal differences in the distribution of repetitive sequences in the genomes of the bacterial endosymbionts. Most of the endosymbionts displayed unique REP-PCR patterns. A cladogram generated from these fingerprints reflected relationships that may be influenced by a variety of factors, including host genera, geographic location, and bottom type.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference51 articles.

1. PCR Methods for Rapid Identification and Characterization of Actinobacillus seminis Strains

2. The 16S/23S ribosomal spacer as a target for DNA probes to identify eubacteria.;Barry T.;PCR Methods Appl.,1991

3. Vertical transmission of a chemoautotrophic symbiont in the protobranch bivalve, Solemya reidi.;Cary S. C.;Mol. Mar. Biol. Biotechnol.,1994

4. Transovarial inheritance of endosymbiotic bacteria in clams inhabiting deep-sea hydrothermal vents and cold seeps.;Cary S. C.;Proc. Natl. Acad. Sci. USA,1993

5. Identification and localization of bacterial endosymbionts in hydrothermal vent taxa with symbiont-specific polymerase chain reaction amplification and in situ hybridization techniques.;Cary S. C.;Mol. Mar. Biol. Biotechnol.,1993

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3