Cloning and Sequencing of an Alkaline Protease Gene from Bacillus lentus and Amplification of the Gene on the B. lentus Chromosome by an Improved Technique-Reference-Cited by-同舟云学术

Cloning and Sequencing of an Alkaline Protease Gene from Bacillus lentus and Amplification of the Gene on the B. lentus Chromosome by an Improved Technique

Published:2000-02 Issue:2 Volume:66 Page:825-827
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Jørgensen Per Linå¹,Tangney Martin²,Pedersen Poul Erik¹,Hastrup Sven¹,Diderichsen Børge¹,Jørgensen Steen T.¹

Affiliation:

1. Bacterial Gene Technology, Novo Nordisk A/S, 2880 Bagsværd, Copenhagen, Denmark,1 and

2. Department of Biological Sciences, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, Scotland, United Kingdom2

Abstract

ABSTRACT A gene encoding an alkaline protease was cloned from an alkalophilic bacillus, and its nucleotide sequence was determined. The cloned gene was used to increase the copy number of the protease gene on the chromosome by an improved gene amplification technique.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.66.2.825-827.2000

Reference13 articles.

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5. Cloning of aldB, which encodes alpha-acetolactate decarboxylase, an exoenzyme from Bacillus brevis

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