Expression, purification, and molecular characterization of a full-length thermostable alkaline protease gene from Bacillus subtilis DMA-09

Author:

Bukhari Dilara Abbas,Barkat Amina,Rehman Abdul

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Plant Science,Genetics,Molecular Biology,Animal Science and Zoology,Biochemistry,Ecology, Evolution, Behavior and Systematics

Reference46 articles.

1. Abbasi-Hosseini SM, Eftekhar F, Yakhchali B, Minai-Tehrani D (2011) Cloning and enhanced expression of an extracellular alkaline protease from a soil isolate of Bacillus clausii in Bacillus subtilis. Iranian J Biotechnol 9(4): 275–280. http://www.ijbiotech.com/article_7129_45ea7dbb7127ac72c4ecbf2eae9cf6f0.pdf

2. Adinarayana K, Ellaiah P, Prasad DS (2003) Purification and characterization of thermostable serine alkaline protease from a newly isolated Bacillus subtilis PE-11. Pharma Sci Technol 4(4):1–9. https://doi.org/10.1208/pt040456

3. Akram M, Shafaat S, Bukhari DA, Rehman, A (2014) Characterization of a thermostable alkaline protease from Staphylococcus aureus S-2 isolated from chicken waste. Pakistan J Zool 46(4): 1125–1132. http://zsp.com.pk/pdf46/1125-1132%20(31)%20PJZ-1845-14%2015-7-14%20Manuscript%20PJZ-14%20(Page%20proof).pdf

4. Alikhajeh J, Khajeh K, Naderi-Mnesh M, Ranjbar B, Sajedi RH, Naderi-Mnesh H (2007) Kinetic analysis, structural studies and prediction of pKa values of Bacillus KR-8104 alpha amylase. The determinants of pH activity profile. Enzyme Microbiol Technol 41:337–345. https://doi.org/10.1016/j.enzmictec.2007.02.019

5. Annamalai N, Rajeswari MV, Sahu SK, Balasubramanian T (2014) Purification and characterization of solvent stable, alkaline protease from Bacillus firmus CAS 7 by microbial conversion of marine wastes and molecular mechanism underlying solvent stability. Process Biochem 49(6):1012–1019. https://doi.org/10.1016/j.procbio.2014.03.007

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