Sau-PCR, a Novel Amplification Technique for Genetic Fingerprinting of Microorganisms-Reference-Cited by-同舟云学术

Sau-PCR, a Novel Amplification Technique for Genetic Fingerprinting of Microorganisms

Published:2005-10 Issue:10 Volume:71 Page:6401-6406
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Corich Viviana¹,Mattiazzi Alessandro¹,Soldati Elisa¹,Carraro Angela¹,Giacomini Alessio¹

Affiliation:

1. Dipartimento di Biotecnologie Agrarie, Università di Padova, Legnaro, Padova, Italy

Abstract

ABSTRACT The proposed technique is based on the digestion of genomic DNA with the restriction endonuclease Sau3AI and subsequent amplification with primers whose core sequence is based on the Sau3AI recognition site. The method was tested on strains of lactic acid bacteria but could be proposed for virtually any culturable organism from which DNA can be extracted.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.71.10.6401-6406.2005

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