Formaldehyde-Inactivated Whole-Virus Vaccine Protects a Murine Model of Enterovirus 71 Encephalomyelitis against Disease-Reference-Cited by-同舟云学术

Formaldehyde-Inactivated Whole-Virus Vaccine Protects a Murine Model of Enterovirus 71 Encephalomyelitis against Disease

Published:2010-01 Issue:1 Volume:84 Page:661-665
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Ong Kien Chai¹,Devi Shamala²,Cardosa Mary Jane³,Wong Kum Thong⁴

Affiliation:

1. Departments of Molecular Medicine

2. Medical Microbiology

3. Institute of Community Health and Medicine, University of Malaysia, Sarawak, Kota Samarahan, Malaysia

4. Pathology, Faculty of Medicine, University of Malaya, Kuala Lumpur

Abstract

ABSTRACT Enterovirus 71 (EV71) causes childhood hand, foot, and mouth disease and neurological complications, and no vaccines or therapeutic drugs are currently available. Formaldehyde-inactivated whole-virus vaccines derived from EV71 clinical isolates and a mouse-adapted virus (MAV) were tested in a mouse model of EV71 encephalomyelitis. After only two immunizations, given to mice at 1 and 7 days of age, the MAV vaccine protected mice at 14 days of age from disease. Tissues from immunized mice were negative for virus by viral culture, reverse transcriptase PCR, immunohistochemistry analysis, and in situ hybridization. Cross-neutralizing EV71 antibodies to strains with genotypes B3, B4, and C1 to C5 generated in immunized adult mice were able to passively protect 14-day-old mice from disease.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.00999-09

Reference28 articles.

1. Alexander, J. P., L. Baden, M. A. Pallansch, and G. Anderson. 1994. Enterovirus 71 infections and neurologic disease—United States, 1977-1991. J. Infect. Dis.169:905-908.

2. Cardosa, M. J., D. Perera, B. A. Brown, D. Cheon, H. M. Chan, K. P. Chan, H. Cho, and P. McMinn. 2003. Molecular epidemiology of human enterovirus 71 strains and recent outbreaks in the Asia-Pacific region: comparative analysis of the VP1 and VP4 genes. Emerg. Infect. Dis.9:461-468.

3. Chan, K. P., K. T. Goh, C. Y. Chong, E. S. Teo, G. Lau, and A. E. Ling. 2003. Epidemic hand, foot, and mouth disease caused by human enterovirus 71, Singapore. Emerg. Infect. Dis.9:78-85.

4. Chan, L. G., U. D. Parashar, M. S. Lye, F. G. L. Ong, S. R. Zaki, J. P. Alexander, K. K. Ho, L. L. Han, M. A. Pallansch, A. B. Suleiman, M. Jegathesan, and L. J. Anderson. 2000. Deaths of children during an outbreak of hand, foot, and mouth disease in Sarawak, Malaysia: clinical and pathological characteristic of the disease. Clin. Infect. Dis.31:678-683.

5. Chen, H. L., J. Y. Huang, T. W. Chu, T. C. Tsai, C. M. Hung, C. C. Lin, F. C. Liu, L. F. Wang, Y. J. Chen, M. F. Lin, and C. M. Chen. 2008. Expression of VP1 protein in the milk of transgenic mice: a potential oral vaccine protects against enterovirus 71 infection. Vaccine26:2882-2889.

Cited by 110 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Substitution of Coxsackievirus A16 VP1 BC and EF Loop Altered the Protective Immune Responses in Chimera Enterovirus A71;Vaccines;2023-08-14

2. Humoral and cellular immunogenicity and efficacy of a coxsackievirus A10 vaccine in mice;Emerging Microbes & Infections;2023-01-17

3. Spectral (FT-IR, FT-Raman, NMR, UV–vis), electronic structure (DFT, TD-DFT), and molecular docking investigations on 1-((1H-benzo[d]imidazol-1-yl)methyl)urea – A bioactive Mannich base system;Chemical Physics Letters;2022-11

4. Pathological Characteristics of Echovirus 30 Infection in a Mouse Model;Journal of Virology;2022-05-11

5. Review: Vaccine Myth-Buster – Cleaning Up With Prejudices and Dangerous Misinformation;Frontiers in Immunology;2021-06-10