The Susceptibility of Mycobacterium tuberculosis to Isoniazid and the Arg→Leu Mutation at Codon 463 of katG Are Not Associated-Reference-Cited by-同舟云学术

The Susceptibility of Mycobacterium tuberculosis to Isoniazid and the Arg→Leu Mutation at Codon 463 of katG Are Not Associated

Published:2001-04 Issue:4 Volume:39 Page:1591-1594
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

van Doorn H. R.¹,Kuijper E. J.¹,van der Ende A.¹,Welten A. G. A.²,van Soolingen D.³,de Haas P. E. W.³,Dankert J.¹

Affiliation:

1. Academic Medical Center, Department of Medical Microbiology,1 and

2. Free University of Amsterdam, Department of Cell Biology, Faculty of Medicine,2 Amsterdam, and

3. National Institute of Public Health and Environment, Diagnostic Laboratory for Infectious Disease and Perinatal Screening, Bilthoven,3 The Netherlands

Abstract

ABSTRACT A mutation (CCG→CTG [Arg→Leu]) in codon 463 of katG (catalase peroxidase) of Mycobacterium tuberculosis has been found in isoniazid (INH)-resistant strains. A PCR restriction endonuclease analysis to detect this mutation was applied to 395 M. tuberculosis isolates from patients in The Netherlands. The proportion of isolates with a detectable mutation was 32% (32 out of 100) and 29% (85 out of 295) among INH-susceptible isolates and INH-resistant or -intermediate isolates, respectively. Sequencing of five INH-susceptible isolates with such mutations showed that all five had the Arg463Leu mutation. We conclude that the Arg463Leu mutation of katG of M. tuberculosis is not a reliable indicator of INH resistance.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.39.4.1591-1594.2001

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