Affiliation:
1. Department of Agricultural Chemistry, University of Tokyo, Bunkyo-ku, Tokyo 113, and Kao Institute for Fundamental Research, Kao Corporation, Ichikai-machi, Haga-gun, Tochigi 321-34,2 Japan
Abstract
Previously, a thermophilic obligate methane-oxidizing bacterium, H-2 (type I), was isolated in our laboratory. H-2 is a new type of methylotroph because of the G+C content of DNA; it uses both the ribulose monophosphate pathway and the serine pathway for carbon assimilation and possesses a new quinone. In addition, we found that resting cell suspensions of H-2 had the ability to oxidize a variety of compounds different from the other methane-oxidizing bacteria as follows. (i) C
1
to C
8
n
-alkanes are hydroxylated and further oxidized, yielding mixtures of the corresponding alcohols, aldehydes, acids, and ketones. Liquid alkanes are transformed through a different oxidative pathway from that of gaseous ones. (ii) Both gaseous (C
2
to C
4
) and liquid (C
5
, C
6
)
n
-alkenes are oxidized to their corresponding 1,2-epoxides. (iii) Liquid monochloro and dichloro
n
-alkanes (C
5
, C
6
) are oxidized, yielding their corresponding acids or haloacids. (iv) Diethyl ether is oxidized to acetic acid; no ethanol and acetaldehyde are detected. (v) Cyclic and aromatic compounds are also oxidized. (vi) Secondary alcohols (C
3
to C
10
) are oxidized to their corresponding methyl ketones.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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