Isolation and Characterization of a Novel Lysine Racemase from a Soil Metagenomic Library-Reference-Cited by-同舟云学术

Isolation and Characterization of a Novel Lysine Racemase from a Soil Metagenomic Library

Published:2009-08 Issue:15 Volume:75 Page:5161-5166
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Chen I-Chien¹,Lin Wei-De¹,Hsu Shin-Kuang²,Thiruvengadam Venkatesan¹,Hsu Wen-Hwei³

Affiliation:

1. Institute of Molecular Biology, College of Life Sciences, National Chung Hsing University, Taichung, Taiwan 402

2. Department of Medical Research, China Medical University Hospital, Taichung, Taiwan 404

3. Department of Dental Laboratory Technology, Central Taiwan University of Science and Technology, Taichung, Taiwan 406

Abstract

ABSTRACT A lysine racemase ( lyr ) gene was isolated from a soil metagenome by functional complementation for the first time by using Escherichia coli BCRC 51734 cells as the host and d -lysine as the selection agent. The lyr gene consisted of a 1,182-bp nucleotide sequence encoding a protein of 393 amino acids with a molecular mass of about 42.7 kDa. The enzyme exhibited higher specific activity toward lysine in the l -lysine-to- d -lysine direction than in the reverse reaction.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00074-09

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