Styrene metabolism in Exophiala jeanselmei and involvement of a cytochrome P-450-dependent styrene monooxygenase-Reference-Cited by-同舟云学术

Styrene metabolism in Exophiala jeanselmei and involvement of a cytochrome P-450-dependent styrene monooxygenase

Published:1996-04 Issue:4 Volume:62 Page:1471-1474
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Cox H H¹,Faber B W¹,Van Heiningen W N¹,Radhoe H¹,Doddema H J¹,Harder W¹

Affiliation:

1. Department of Environmental Biotechnology, TNO Institute of Environmental Sciences, Energy Research and Process Innovation, JA Delft, Netherlands.

Abstract

The yeast-like fungus Exophiala jeanselmei degrades styrene via initial oxidation of the vinyl side chain to phenylacetic acid, which is subsequently hydroxylated to homogentisic acid. The initial reactions are catalyzed by a NADPH- and flavin adenine dinucleotide-dependent styrene monooxygenase, a styrene oxide isomerase, and a NAD(+)-dependent phenylacetaldehyde dehydrogenase. The reduced CO-difference spectrum of microsomal preparations of styrene-grown cells shows a characteristic absorption maximum at 450 nm, which strongly suggests the involvement of a cytochrome P-450-dependent styrene monooxygenase. Inhibition of styrene monooxygenase activity in cell extracts by cytochrome P-450 inhibitors SKF-525-A, metyrapone, and CO confirms this assumption.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.62.4.1471-1474.1996

Reference26 articles.

1. Berdowski J. J. M. and W. J. Jonker. 1993. Industrial emissions in The Netherlands; industrial sectors individual compounds and distribution over regions. Emission registration publication series 5th inventory-1990. Ministry of Housing Physical Planning and the Environment The Hague The Netherlands. (In Dutch.)

2. Metabolism of naphthalene by cell extracts of Cunninghamella elegans;Cerniglia C. E.;Arch. Biochem. Biophys.,1978

3. Chapman P. J. 1979. Degradation mechanisms p. 28-66. In A. W. Bourquin and P. H. Pritchard (ed.) Proceedings of the Workshop on Microbial Degradation of Pollutants in Marine Environments Gulf Breeze Florida. EPA600/9-79-012. U.S. Environmental Protection Agency Washington D.C.

4. Cox H. H. J. 1995. Styrene removal from waste gas by the fungus Exophiala jeanselmei in a biofilter. Ph.D. thesis. University of Groningen Groningen The Netherlands.

5. Cox H. H. J. H. J. Doddema and W. Harder. 1994. Biological removal of styrene from waste gases using fungi on inert support materials p. 289-299. In VDI-Berichte 1104. VDI Verlag Düsseldorf Germany.

Cited by 63 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Biodegradation of Polystyrene by Galleria mellonella: Identification of Potential Enzymes Involved in the Degradative Pathway;International Journal of Molecular Sciences;2024-01-27

2. An investigation into a petrochemical effluent treatment using a membrane biological reactor;Environment, Development and Sustainability;2023-08-26

3. Styrene Oxide Isomerase‐Catalyzed Meinwald Rearrangement in Cascade Biotransformations: Synthesis of Chiral and/or Natural Chemicals;Chemistry – A European Journal;2023-03-20

4. Understanding the in silico aspects of bacterial catabolic cascade for styrene degradation;Proteins: Structure, Function, and Bioinformatics;2022-11-30

5. Ten decadal advances in fungal biology leading towards human well-being;Fungal Diversity;2022-09