An N-Linked Glycoprotein with α(2,3)-Linked Sialic Acid Is a Receptor for BK Virus

Author:

Dugan Aisling S.12,Eash Sylvia12,Atwood Walter J.2

Affiliation:

1. Graduate Program in Pathobiology

2. Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island

Abstract

ABSTRACT BK virus (BKV) is a common human polyomavirus infecting >80% of the population worldwide. Infection with BKV is asymptomatic, but reactivation in renal transplant recipients can lead to polyomavirus-associated nephropathy. In this report, we show that enzymatic removal of α(2,3)-linked sialic acid from cells inhibited BKV infection. Reconstitution of asialo cells with α(2,3)-specific sialyltransferase restored susceptibility to infection. Inhibition of N-linked glycosylation with tunicamycin reduced infection, but inhibition of O-linked glycosylation did not. An O-linked-specific α(2,3)-sialyltransferase was unable to restore infection in asialo cells. Taken together, these data indicate that an N-linked glycoprotein containing α(2,3)-linked sialic acid is a critical component of the cellular receptor for BKV.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

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