Rapid determination of antifungal activity by flow cytometry

Author:

Green L1,Petersen B1,Steimel L1,Haeber P1,Current W1

Affiliation:

1. Lilly Research Laboratories, Eli Lilly and Co., Indianapolis, Indiana 46202.

Abstract

We have developed a rapid assay of antifungal activity which utilizes flow cytometry to detect accumulation of a vital dye in drug-damaged fungal cells. Results of these studies suggest that flow cytometry may provide an improved, rapid method for determining and comparing the antifungal activities of compounds with differing modes of action.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference25 articles.

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5. Comparison of relative susceptibilities of Candida species to three antifungal agents as determined by unstandardized methods;Galgiani J. N.;Antimicrob. Agents Chemother.,1987

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