Affiliation:
1. Institute of Biochemistry and Biotechnology, Technical University of Braunschweig, 38106 Braunschweig, Germany
Abstract
ABSTRACT
The fungus
Fusarium oxysporum
produced a
d
-amino acid oxidase (EC
1.4.3.3
) in a medium containing glucose as the carbon and energy source and ammonium sulfate as the nitrogen source. The specific
d
-amino acid oxidase activity was increased up to 12.5-fold with various
d
-amino acids or their corresponding derivatives as inducers. The best inducers were
d
-alanine (2.7 μkat/g of dry biomass) and
d
-3-aminobutyric acid (2.6 μkat/g of dry biomass). The addition of zinc ions was necessary to permit the induction of peroxisomal
d
-amino acid oxidase. Bioreactor cultivations were performed on a 50-liter scale, yielding a volumetric
d
-amino acid oxidase activity of 17 μkat liter
−1
with
d
-alanine as an inducer. Under oxygen limitation, the volumetric activity was increased threefold to 54 μkat liter
−1
(3,240 U liter
−1
).
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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