Molecular Fingerprinting of Mycobacterium bovis subsp. caprae Isolates from Central Europe

Author:

Erler Wilfried1,Martin Gerald1,Sachse Konrad1,Naumann Ludmila23,Kahlau Dagmar3,Beer Jörg4,Bartos Milan5,Nagy György6,Cvetnic Zeljko7,Zolnir-Dovc Manca8,Pavlik Ivo5

Affiliation:

1. Federal Research Centre for Virus Diseases of Animals (BFAV), Jena

2. Institute of Medical Microbiology and Hygiene, University of Regensburg, Regensburg

3. Bavarian State Office for Health and Food Safety, Oberschleissheim

4. Institute of Medical Microbiology, University of Leipzig, Leipzig

5. Germany; Veterinary Research Institute, Brno, Czech Republic

6. Central Veterinary Institute, Budapest, Hungary

7. Croatian Veterinary Institute, Zagreb, Croatia

8. University Clinic of Respiratory and Allergic Diseases, Golnik, Slovenia

Abstract

ABSTRACT To study the dissemination of Mycobacterium bovis subsp. caprae , 79 European isolates from cattle, humans, and other hosts were examined by spoligotyping and IS 6110 restriction fragment length polymorphism (RFLP) analysis. Among a total of 11 different spoligotypes identified, type C1 proved to be predominant ( n = 62). Five of the spoligotypes are described for the first time. A total of 43 different RFLP types were identified, thus allowing further differentiation for epidemiological tracking. Isolates from a series of outbreaks in one village proved to be of the same spoligotype and of identical or closely related RFLP types.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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