Comparison of VITEK 2 with ITS2-Fragment Length Polymorphism Analysis for Identification of Yeast Species-Reference-Cited by-同舟云学术

Comparison of VITEK 2 with ITS2-Fragment Length Polymorphism Analysis for Identification of Yeast Species

Published:2004-05 Issue:5 Volume:42 Page:2209-2211
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Massonet Caroline¹,Van Eldere Johan¹²,Vaneechoutte Mario³,De Baere Thierry³,Verhaegen Jan²,Lagrou Katrien²

Affiliation:

1. Department of Immunology and Microbiology, Rega Institute

2. Laboratorium geneeskunde, U.Z. Gasthuisberg, B-3000 Leuven

3. Department of Clinical Microbiology and Immunology, Ghent University Hospital, B-9000 Ghent, Belgium

Abstract

ABSTRACT A total of 61 clinical yeast isolates of Candida , Cryptococcus , Blastoschizomyces , and Saccharomyces spp. were used to compare two identification techniques, VITEK 2 and ITS2-fragment length polymorphism analysis (ITS2-FLP), with ID32C as the reference method. ID32C identified 58 isolates correctly. ITS2-FLP with Instagene DNA extraction identified 59 isolates. ITS2-FLP combined with boiling-freezing DNA extraction identified 55 isolates. VITEK 2 identified 41 isolates correctly.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.5.2209-2211.2004

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